HPLC Columns and Cartridges
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TSKgel™ Super-ODS HPLC Column, 2.3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ Super-ODS Columns are packed with monodispersed, 2 μm (nominal, mean particle size is 2.3 micron) spherical silica particles covalently bonded with phenyl groups.
| Shipping Condition | Shipped in 70% Methanol, 30% Water |
|---|---|
| Particle Size | 2.3 μm |
| Solvents | 0-100% Organic Solvent |
| Carbon Load | 8% |
| Stationary Phase | C18 (octadecyl) |
| Temperature | 10°C to 50°C |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase/Silica Particle Platform |
| Endcapped | Yes |
| pH | 2 to 7.5 |
| Surface Area | 100 m2/g |
| Product Line | TSKgel |
| USP Type | L1 |
Thermo Scientific™ POROS™ GoPure™ 50 HQ Pre-packed Column, 0.8 x 10 cm, 5 mL
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Thermo Scientific™ POROS™ GoPure™ 0.8 cm pre-packed columns are intended for the screening of POROS affinity, ion exchange, and hydrophobic interaction chromatography (HIC) resins or for small scale purification.
Thermo Scientific™ Hypersil GOLD™ C4 HPLC Columns
Featuring a lower hydrophobicity than C18 or C8 columns, Thermo Scientific™ Hypersil GOLD™ C4 columns provide outstanding sensitivity and high efficiency.
| Pore Size | 175 Å |
|---|---|
| Carbon Load | 5% |
| Stationary Phase | C4 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| pH | 2 to 8 |
| Surface Area | 220 m²/g |
| USP Type | L26 |
Thermo Scientific™ BetaSil™ Phenyl/Hexyl Columns, 5μm Particle Size
Thermo ScientificTM BetaSilTM Phenyl/Hexyl Columns are available in limited dimensions. Please contact Customer Service for more details.
| Pore Size | 100 Å |
|---|---|
| Stationary Phase | Phenyl/Hexyl |
| Phase | Reversed Phase |
| For Use With | LC/MS |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| Column Format | Analytical Column |
| Particle Size | 5 μm |
| Carbon Load | 11% |
| Packing Material | Silica |
| Diameter (Metric) | 4.6 mm |
| pH | 2 to 9 |
| Product Line | Beta |
| USP Type | L11 |
MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18 HPLC Column, 3 μm Particle Size
A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.
| Pore Size | 120 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | C18 (Octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (Octadecyl) Phase/Silica Gel, Spherical Particle Platform |
| Endcapped | Yes |
| Surface Area | 170 m2/g |
| Product Line | SUPELCOSIL |
TSKgel™ G2000SWXL HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel™ SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
MilliporeSigma™ Supelco™ Ascentis™ Express HILIC UHPLC Column, 2 μm Particle Size
Contains a bare silica stationary phase that can be used for traditional normal phase separations or aqueous normal phase chromatography.
| Pore Size | 90 Å |
|---|---|
| Particle Size | 2 μm |
| Stationary Phase | Silica |
| Phase | Reversed Phase |
| Matrix | Silica Phase/Fused-Core Particle Platform |
| Endcapped | No |
| Technique | Fused-Core UHPLC |
| Product Line | Ascentis |
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 5 μm Particle Size
Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.
| Pore Size | 300 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | C5 (pentyl) |
| Phase | Reversed Phase |
| Matrix | C5 (pentyl) Phase/Silica Particle Platform |
| Endcapped | Yes |
| Technique | Wide Pore HPLC |
| Surface Area | 100 m2/g |
| Product Line | Discovery |
Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns
Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.
| Shipping Condition | Wet Ice |
|---|---|
| Pore Size | 2000 Å |
| Content And Storage | Column includes EZ grip fittings and column test certificate Store at 4°C. DO NOT FREEZE. |
| Stationary Phase | Human Serum Albumin affinity ligand, humanspecific |
| Column Type | Affinity |
| Quantity | 1 Column |
| Material | PEEK |
| High-throughput Compatibility | Not High-throughput Compatible (Manual) |
| Max. Pressure | 180 bar |
| Particle Size | 20 μm |
| Flow Rate | Up to 5000 cm/hr. |
| Type | Column |
| Matrix | Polystyrenedivinylbenzene |
| Reactivity | Human |
| Target Molecule | HSA, HSA Fusion Proteins |
| Binding Capacity | 2.6 mg/mL |
| Sample Type | Cell Culture Supernatants |
| Purification Target | HSA, HSA Fusion Proteins |
| Product Line | CaptureSelect, POROS |
TSKgel™ IC-Cation SW HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ IC-Cation SW is a high performance ion chromatography column used without a suppression system.
MilliporeSigma™ Supelco™ Hamilton PRP-C18 HPLC Column, 5 μm Particle Size, Stainless Steel
The Hamilton PRP-C18 HPLC column is designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase |
| Material | Stainless Steel |
| Product Line | Hamilton |
Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Columns
Separate challenging peptide mapping samples with Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns.
| Pore Size | 100 Å |
|---|---|
| Stationary Phase | C18 |
| Temperature | 60°C |
| Phase | Reversed Phase |
| Endcapped | Yes |
| Column Type | Reversed Phase |
| For Use With (Application) | Bottom-up Proteomics, LC-MS |
| Surface Area | 450 m2/g |
| Max. Pressure | 22,000 psi (1500 bar) |
| Column Format | Analytical Column, EASY-Spray™ |
| Particle Size | 2 μm |
| Carbon Load | 15% |
| Packing Material | Spherical, Fully Porous, Ultrapure Silica |
| Diameter (Metric) | 75 μm |
| pH | 2 to 8 |
| Product Line | Easy Spray |
| USP Type | L1 |
Thermo Scientific™ Accucore™ Biphenyl Reversed Phase HPLC Columns
Use this solid core column for fast and reliable separation of critical pairs and isomers.
| Pore Size | 80 Å |
|---|---|
| Stationary Phase | Biphenyl |
| Temperature | 70°C |
| Phase | Reversed Phase |
| For Use With | UHPLC |
| Endcapped | Yes |
| Surface Area | 130 m²/g |
| Max. Pressure | 8700 psi (600 bar) |
| Particle Size | 2.6 μm |
| Carbon Load | 6% |
| Packing Material | Ultrapure Silica |
| Diameter (Metric) | 2.1 mm |
| pH | 2 to 8 |
| Particle Shape | Solid Core |
| Product Line | Accucore |
| USP Type | L11 |
MilliporeSigma™ Supelco™ TSKgel™ G2500HXL HPLC Column, 5 μm Particle Size
TSKgel G2500HXL Columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of oligomers and polymers of less than 20,000 Da.
MilliporeSigma™ Supelco™ BIOshell™ A160 Peptide C18 HPLC Column, 160 Å, 4.7 μm Particle Size
BIOshell™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.
| Pore Size | 160 Å |
|---|---|
| Particle Size | 4.7 μm |
| Carbon Load | 4% |
| Stationary Phase | C18 (octadecyl) |
| Phase | Reversed Phase |
| Matrix | C18 (octadecyl) Phase/Fused-Core Particle Platform |
| Endcapped | No |
| pH | 1 to 8 |
| Material | Stainless Steel |
| Technique | Fused-Core HPLC |
| Surface Area | 60 m2/g |
| Product Line | BIOshell |